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Cell line: | N1E-115-1 |
DSMZ no.: | ACC 856 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | neuroblastoma |
Origin: | Subclone of N1E-115, an adrenergic cell line established from mouse strain A/Jax with neuroblastoma C1300 |
Reference(s): | 14354, 18176 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | mostly round adherent cells growing in monolayers or small clumps, occasionally forming spindle-like extensions |
Medium: | 80-90% DMEM (with 25 mM HEPES) + 10-20% h.i. FBS |
Subculture: | seed out at 0.25 x 106 cells/25 cm2; viability is rather low after thawing, but cells recover soon, when medium is exchanged every second day; culture does not grow to complete confluency; split sub confluent culture 1:2-1:4 once a week using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 100 hours |
Harvest: | cell harvest of ca. 1.5 x 106 cells/80 cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Viruses: | PCR: MLV+ |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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