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| Cell line: | NT-27a |
| DSMZ no.: | ACC 857 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | hepatocellular tumor |
| Origin: | subclone of NT-27 which was established ca. 1986 from the liver of a male Wistar AF/Han rat treated with N-methyl-N-nitrosourea |
| Reference(s): | 18184, 18185, 14658, 15145 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently in clusters as monolayer |
| Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/175 cm2; split confluent culture 1:5 to 1:7 once a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 24-30 hours |
| Harvest: | cell harvest of ca. 25 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Viruses: | PCR: MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
