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Cell line: | CHO-INSR-1284 |
DSMZ no.: | ACC 858 |
Species: | Chinese hamster (Cricetulus griseus) |
Cell type: | ovary cells |
Origin: | Flp-In derivate of CHO-K1 cell line (originated from an ovary biopsy of an adult Chinese hamster in 1957) which were transfected with the gene for human insulin receptor B (INSR) inserted in the vector pCDNA(TM)5-FRT-TO from Invitrogen |
Reference(s): | 18190, 14860 |
Biosafety level: | 1, GMO-S1 |
Risk assessment: | genetically modified organism, according to German and European legislation allocated into group S1 |
Permissions and restrictions: | A, D |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid-like cells growing adherently in monolayers |
Medium: | 90% Ham´s F12 + 10% h.i. FBS + 1 mM L-glutamine + 0.3 mg/ml Hygromycin |
Subculture: | seed out 0.5-1 x 106 cells/80cm2; split 1:4 to 1:6 once or twice a week using accutase; after thawing viability may decrease and cells may need a few days to recover |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | cell harvest of ca. 8-10 x 106 cells/80 cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD220 +; image |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Cricetulus griseus species. |
Viruses: | PCR: MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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