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| Cell line: | FAIK3-5 |
| DSMZ no.: | ACC 861 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | kidney |
| Origin: | REP (renal Epo-producing) cells from the kidney of adult transgenic female mice (B6D2;C57BL6N-Tg(EPO::Cre)1RHW x
B6.Cg-Gt(ROSA)26Sor/J), immortalized with SV-40 large-T antigen. Cells are described to produce Epo under hypoxia (but no expression under normoxic conditions). Resistant to puromycin up to 0.5 µg/ml. |
| Reference(s): | 18189 |
| Biosafety level: | 1, GMO-S1 |
| Risk assessment: | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions: | A, D |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | flat, partially round, spindle and polygonal (fibroblastoid, telocyte-like) cells growing adherently in monolayers |
| Medium: | 90-95% DMEM + 5-10% h.i. FBS |
| Subculture: | seed out 0.5-1 x 106 cells/80 cm2;
split 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2, normal oxygen level |
| Doubling time: | about 20-30 hours |
| Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Viruses: | PCR: MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
