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Cell line: | UPCI-SCC-003 |
DSMZ no.: | ACC 863 |
Species: | human (Homo sapiens) |
Cell type: | squamous cell carcinoma |
Origin: | established in 1992 from a new primary tumor of the tonsils (grade 1, T1N0) of a 65-year-old woman with head and neck squamous cell carcinoma |
Reference(s): | 15061, 14735, 15176 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004). |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing adherently in monolayers; image; image; image |
Medium: | 90% DMEM + 10% h.i. FBS + 2 mM L-glutamine + 1% sodium pyruvate +1% non-essential amino acids |
Subculture: | seed out at about 1 x 106 cells/80 cm2;
split semi-confluent culture up to 1:10 once a week using TripleExpress. Flasks coated with 0.01% poly-L-lysine may be used if the cells do not adhere |
Incubation: | at 37 °C with 5-10% CO2 |
Doubling time: | ca. 45 hours |
Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | to make inquiries about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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