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| Cell line: | UPCI-SCC-078 |
| DSMZ no.: | ACC 867 |
| Species: | human (Homo sapiens) |
| Cell type: | squamous cell carcinoma |
| Origin: | established in 1994 from a recurrent oral squamous cell carcinoma (stage 2, T2N0) of the mouth cavity of a 60-year-old man after radiation and chemotherapy. Described to carry a chromosomal 11q13 amplification and a loss of function mutation of TP53 (codon 158: Arg-Gly). The cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-414-648-4004) at the University of Pittsburgh, Office of Technology Management |
| Reference(s): | 15061, 14735, 18197, 18198, 18199 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004). |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently as monolayer; image; image |
| Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS + non-essential amino acids + 2 mM L-glutamine |
| Subculture: | seed out at about 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 3-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60 hours |
| Harvest: | cell harvest of ca. 5 x 106 cells/80 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | to make inquiries about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -,
HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
