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| Cell line: | GDM-1 |
| DSMZ no.: | ACC 87 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myelomonocytic leukemia |
| Origin: | established in 1979 from the peripheral blood of a 66-year-old woman with a Philadelphia chromosome negative myeloproliferative disorder (resembling chronic myeloid leukemia, CML, in blast crisis) after transformation to refractory acute myelomonocytic leukemia (AML) |
| Reference(s): | 14378 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single round to oval cells in suspension; image; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-0.8 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days (starting the culture in a 12- or 24-well plate may be of advantage) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | maximum density at 1.2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD4 +, CD13 -, CD14 -, CD15 (+), CD19 -, CD33 +, CD34 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ASN-0002.1 resulted in an authentic STR profile of the |
| Cytogenetics: | human hyperdiploid karyotype - 48(46-48)<2n>XX, +8, +13, -16, +mar, t(2;11)(q36;q13), del(6)(q21), t(7;?)(q35;?), del(12)(p13) |
| Molec. Genetics: | expression of MNX1 (HLXB9) confirmed by RT-PCR and Western blot |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
