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| Cell line: | THB-5 |
| DSMZ no.: | ACC 88 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-C3d receptor and anti-EBV receptor) |
| Origin: | established by fusion of the P3X63AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse immunized with human B cell line SB; cells were reported to produce a monoclonal antibody (IgG2akappa) reacting with the human C3d receptor (CD21) and an EBV receptor of 145 kDa |
| Reference(s): | 15056 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, round cells in suspension; image; image; image; image |
| Medium: | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture: | optimal density at 0.5 x 106 cells/ml, minimal density at 0.1 x 106 cells/ml; split 1:3 to 1:5; after thawing, it is advised to use feeder cells for the first 7-10 days; seed out at ca. 0.5-1.0 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 50 hours |
| Harvest: | maximal density at 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | secretion of a IgG2a monoclonal antibody |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
