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Cell line: | DLRP |
DSMZ no.: | ACC 881 |
Species: | human (Homo sapiens) |
Cell type: | lung squamous cell carcinoma |
Origin: | established in 1989 from a metastasis of a cervical lymph node of a 61-year-old male with a poorly differentiated squamous cell carcinoma of the lung |
Reference(s): | 18222 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing adherently as monolayer; image; image |
Medium: | DMEM + Ham's F12 (1:1) + 10-20% h.i. FBS |
Subculture: | seed out at about 0.5 x 106 cells in a 25 cm2 flask; split 1:2 once a week using trypsin/EDTA; optimal cell density 1-2 x 106 cells/80 cm2; cells grow very slowly |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 1 week |
Harvest: | cell harvest of ca. 3 x 106 cells/80 cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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