| | |
|---|
| Cell line: | UPCI-SCC-182 |
| DSMZ no.: | ACC 887 |
| Species: | human (Homo sapiens) |
| Cell type: | squamous cell carcinoma |
| Origin: | established in 1996 from a primary, moderately differentiated oral squamous cell carcinoma (stage 2, T2N1) of the oral retromolar trigone of a 71-year-old man.
Described as HPV negative and to carry a TP53 mutation (E294X), but no chromosomal 11q13 amplification |
| Reference(s): | 15061, 15176, 18223 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004). |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently in colony-like clusters; image; image; image; image |
| Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 4-8 days using trypsin/EDTA for 5-10 min (before trypsination wash 2x with PBS); initially after thawing cells may grow slowly |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-80 hrs (after recovery from liquid nitrogen ca. 100 hrs) |
| Harvest: | ca. 15 x 106 cells/175 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
