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| Cell line: | 72A1 |
| DSMZ no.: | ACC 89 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-EBV) |
| Origin: | established by fusion of the P3X62AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse immunized with Epstein-Barr virus (EBV) particles; cells produce a monoclonal antibody (IgG1) that reacts with a viral envelope glycoprotein (350/220 kD) and that neutralizes EBV |
| Reference(s): | 14612 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single cells in suspension; image; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | maintain at 0.1-1.0 x 106 cells/ml; split every 2-3 days at 1:3 to 1:5 |
| Incubation: | at 37 °C with 5% CO2 |
| Harvest: | 1.0-1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
