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established from the peripheral blood of a 53-year-old man with IgE-secreting myeloma (refractory, terminal) in 1968; cells were described in the literature to produce IgE lambda; possible fusion partner for hybridoma production; cells express mRNA for BCL2 gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
round to polygonal, single or clustered cells in suspension, some loosely adherent; image; image; image
80-90% RPMI 1640 + 10-20% h.i. FBS
seed out at ca. 0.5-0.7 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; maintain at 0.2-1.0 x 106 cells/ml; cells grow partially (loosely) adherent and can be removed with a bent Pasteur pipette (trypsin is not necessary)
at 37 °C with 5% CO2
ca. 55 hours
saturation density at 1.0-1.5 x 106 cells/ml
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays