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Cell line: | NCI-H209 |
DSMZ no.: | ACC 900 |
Species: | human (Homo sapiens) |
Cell type: | small cell lung carcinoma |
Origin: | established in 1979 from the bone marrow aspirated from a 55-year-old man with small cell lung carcinoma prior to chemotherapy treatment (also named H-209); described as expressing neuroendocrine biochemical markers, e.g. neuron-specific enolase, brain creatine kinase, L-DOPA decarboxylase and bombesin and to be tumorigenic in nude mice;
listed in the CCLE (ref 18232) with extended genome and protein analyses |
Reference(s): | 14421, 14849, 15046, 18232, 40405 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | spherical cells growing as large aggregates; determination of viability and cell number may be difficult; medium may contain large amounts of debris; image; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 3-4 days; cells may grow slowly after thawing |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 60-80 hours |
Harvest: | maximum density of ca. 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Molec. Genetics: | Sequencing confirmed a point mutation of the RB1 gene (codon 706: Cys -> Phe); a wild-typ allele was not detected |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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