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| Cell line: | MaS-3 |
| DSMZ no.: | ACC 901 |
| Species: | human (Homo sapiens) |
| Cell type: | urinary bladder carcinosarcoma |
| Origin: | established in 2018 from tumor tissue of a
86-year-old male with carcinosarcoma of the urinary bladder after neoadjuvant radiotherapy |
| Reference(s): | 18248 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently as monolayer; image; image; image |
| Medium: | 90% RPMI 1640 with 4.5 glucose (5g/l) + 10% h.i. FBS |
| Subculture: | seed out at about 0.5-1 x 106 cells in a 80 cm2 flask; split confluent cukture 1:2 to 1:3 once or twice a week using trypsin/EDTA (wash 2x with PBS and then incubate for 5-10 min with trypsin) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-80 hours |
| Harvest: | cell harvest of ca. 3 x 106 cells/80 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
