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Cell line: | A-431 |
DSMZ no.: | ACC 91 |
Species: | human (Homo sapiens) |
Cell type: | epidermoid carcinoma |
Origin: | established from the solid tumor of an 85-year-old woman; cells were reported to have large numbers of EGF binding sites; cell line was used as indicator cell line for anti-TGF binding |
Reference(s): | 14556 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like, adherent cell line growing in monolayers; image; image; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:4 to 1:6 every 4-5 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 80-100 hours |
Harvest: | about 7-12 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypotriploid karyotype with 24% polyploidy - 64(58-64)<3n>XX/XXX, -2, +3, +7, -8, +10, -11, -13, -14, -15, -16, -18, -22, +2mar - del(3)(p11)x1-2, add(4)(p15), add(6)(p21.3), del(7)(p15), der(7)t(7;11)(p11/13;q11/13)add(11)(q23), add(8)(p21), add(11)(q23), der(14)t(13;14)(q11;p11), add(16)(p13) - carries t(7;11) with breakpoint at EGFR (which it overexpresses) - corresponds to published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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