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| Cell line: | TMM |
| DSMZ no.: | ACC 95 |
| Species: | human (Homo sapiens) |
| Cell type: | B lymphoblastoid cells (from patient with chronic myeloid leukemia in blast crisis) |
| Origin: | established from the peripheral blood of a 62-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) in blast crisis in 1985; cells were described in the literature to be Ph-negative, BCR-ABL1-negative; cells appear to be rather a "normal" B-lymphoblastoid cell line (B-LCL) |
| Reference(s): | 14754 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | polygonal cells, single or in clusters in suspension; image; image; image; image; image |
| Medium: | 90% Iscove's MDM + 10% h.i. FBS |
| Subculture: | seed out at 0.7 x 106 cells/ml; maintain at 0.2-0.8 x 106 cells/ml; split 1:1.5 to 1:2 every 2 to 3 days; the culture usually contains much cell debris |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-40 hours |
| Harvest: | saturation density at about 2.5-4.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD33 -, CD37 +, HLA-DR +, anti-EBNA2 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda - |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype with 8% polyploidy - 46(42-46)<2n>XY - small ring present in 6% cells - no consistent numerical or structural rearrangement - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
