Summary and additional information: | <- T. Clavel, RWTH Aachen; CLA-SR-6. Gut content; wild mouse. Germany, Aachen. Sequence accession no. 16S rRNA gene: OM658561. poposed name: Adlercreutzia agrestimuris
. (Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). , incubation time: 2-3 days, gas phase: 80%N2, 20%CO2, pH 7, 37°C, anaerobic or Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid media add 5% horse blood., incubation time: 2-3 days, gas pack, pH 7, 37°C, anaerobic) |