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BACTERIA | How to read the following data (Example) |
Name: | Polynucleobacter asymbioticus (Hahn et al. 2009) Hahn et al. 2016 |
DSM No.: | 18221, Type strain dsm-18221 dsm18221 dsm 18221 Polynucleobacter asymbioticus |
Strain designation: | QLW-P1DMWA-1 |
Other collection no. or WDCM no.: | CIP 109841 |
Isolated from: | acidic freshwater pond |
Country: | Austria Alps, (47° 44' 27'' N 13° 17' 53'' E at 1300 meters above sea level) |
Date of sampling: | 10/2003 |
Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. |
History: | <- M. W. Hahn <- M. W. Hahn and Q. L. Wu, Austrian Academy of Sciences, Institute of Limnology; QLW-P1DMWA-1 |
Genbank accession numbers: | 16S rRNA gene and and ITS1: AJ879783 complete genome: CP000655 |
Cultivation conditions: | Medium 830 keep agar surface moist with a film of liquid medium, 28°C
Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.
Complete DSMZ Media List |
Summary and additional information: | <- M. W. Hahn <- M. W. Hahn and Q. L. Wu, Austrian Academy of Sciences, Institute of Limnology; QLW-P1DMWA-1. Acidic freshwater pond; Austria, Alps, (47° 44' 27'' N 13° 17' 53'' E at 1300 meters above sea level). Type strain. Taxonomy/description (10512, 13477, 25966, 25987). Sequence accession no. 16S rRNA gene and and ITS1: AJ879783, complete genome: CP000655 (19209). (Medium 830, 28°C). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate. |
Literature: | 10512, 13477, 19209, 25966, 25987 |
Risk group: | 1 (classification according to German TRBA) |
Supplied as: | Delivery form | | Prices | | actively growing culture | | 160,- € | | DNA | | 150,- € | | Price Category for this culture: 1 |
Freight and handling charges will be added. See price list. |
Other cultures: | All DSMZ cultures of the species |
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