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<- E. Leistner, Inst. Pharmazeutische Biologie, Rheinische Friedrich-Wilhelms-Univ., Bonn, Germany; PBB 8. Mutant of E. coli mutant PBB7 (DSM 18415) with neither an intact entC nor an intact menF isochorismate synthase gene. Construction of pbb8 (13425). Role of entC and menF genes in enterobactin and menaquinone biosynthesis (13424). Clustering of isochorismate synthase genes, channeling of isochorismic acid (13423). Produces neither menaquinone (MK8) nor enterobactin (13424). (Medium 381, 37°C).