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| BACTERIA |  How to read the following data (Example) | |||||||||||||||||
| Name: | Polynucleobacter duraquae Hahn et al. 2016 | |||||||||||||||||
| DSM No.: | 21495, Type strain dsm-21495 dsm21495 dsm 21495 Polynucleobacter duraquae | |||||||||||||||||
| Strain designation: | MWH-MoK4, CIP 110977 | |||||||||||||||||
| Other collection no. or WDCM no.: | CIP 110977 | |||||||||||||||||
| Isolated from: | water of freshwater lake | |||||||||||||||||
| Country: | Austria Lake Mondsee (47° 49' 47.11" N 13° 22' 33.94"E) | |||||||||||||||||
| Date of sampling: | 30.09.2002 | |||||||||||||||||
| Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. | |||||||||||||||||
| History: | <- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-MoK4 | |||||||||||||||||
| Genbank accession numbers: | 16S rRNA gene: AJ550654 complete genome: CP007501 | |||||||||||||||||
| Cultivation conditions: | Medium 830 , Prolonged incubation time up to 3-4 weeks., 15°C Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate. Incubation time: more than 14 days Complete DSMZ Media List | |||||||||||||||||
| Summary and additional information: | <- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-MoK4. Water of freshwater lake; Austria, Lake Mondsee (47° 49' 47.11" N 13° 22' 33.94"E). Type strain. Taxonomy/description (25966, 25987). Sequence accession no. 16S rRNA gene: AJ550654, complete genome: CP007501 (25987). (Medium 830, 15°C. Prolonged incubation time up to 3-4 weeks.). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.. | |||||||||||||||||
| Literature: | 25966, 25987 | |||||||||||||||||
| Risk group: | 1 (classification according to German TRBA) | |||||||||||||||||
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Freight and handling charges will be added. See price list. | |||||||||||||||||
| Other cultures: | All DSMZ cultures of the species | |||||||||||||||||
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