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PLASMIDS | |
Name: | pHG27 |
DSM No.: | 8374 dsm-8374 dsm8374 dsm 8374 pHG27 |
History: | <- D. Nies, FU Berlin, strain AH |
Mol. weight: | 200 kb |
Marker: | Resistance to Cu2+, Cd2+, Zn2+, Co2+, Ni2+ |
Remarks: | This strain tolerates a high concentration of copper (MIC = 4 mM CuSO4). DNA from strain AH strongly hybridizes with czc, cnr and chr specific probes cloned from Ralstonia eutropha CH34. The czc-like determinant of strain AH maps on the chromosome, cnr homologs of strain AH map on the chromosome and on the plasmid. The chromate resistance determinant seems to be plasmid borne, but only low chromate levels are tolerated. Strain expresses resistance to HgCl2 (MICs < 1 μM) but not to phenylmercuric acetate. The Hgr determinant of this strain shows homology to the mer determinant of Tn21 on plasmid pDU1003. |
Distributed in: | Cupriavidus metallidurans (former Alcaligenes denitrificans) AH |
Cultivation conditions: | Medium 660, 30°C |
Literature: | 6247 |
Risk group: | 1 (classification according to German TRBA) |
Restrictions: | no |
Country: | country of origin unknown |
Date of sampling: | before 20.02.1995 |
Nagoya Protocol Restrictions: | There are NO known Nagoya protocol restrictions for this strain. |
Supplied as: | Delivery form | | Organization type | | | | Profit | | Non-Profit | | | Freeze Dried | | 100,- € | | 100,- € | | Active culture on request | | 240,- € | | 240,- € | |
Price category 1 strain. Please see price list for freight and handling charges |
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