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PHAGES | |
Species: | Escherichia phage U3 |
Isolate: | Escherichia phage U3 |
DSM No.: | 8986 dsm-8986 dsm8986 dsm 8986 |
History: | <- M. Ott, Univ. Würzburg <- G. Schmidt, Borstel |
Isolated from: | raw sewage |
Geographic origin: | country of origin unknown |
Date of sampling: | before 10.02.1994 |
Nagoya Protocol Restrictions: | There are NO known Nagoya protocol restrictions for this strain. |
Life cycle: | virulent phage, lytic cycle |
Plaques description: | clear and large, about 6 mm in diameter after overnight incubation at 37°C |
Image: | ###IMAGE_TPL### Single plaques on agar plate. Creator: B. Henze, Leibniz Institute DSMZ. © © Leibniz-Institut DSMZ |
Further information: | Mutants blocked in the synthesis of uridine diphosphogalactose are resistant, because they lack phage receptor sites.
This phage is highly specific for and does only attack E. coli K12 (Gal+) and is therefore a useful identification tool for K12 whereas strains B, C or W are not sensitive |
Literature: | 6272 |
Risk group: | 1 |
Phage propagation | |
Host bacterium: | Escherichia coli (Migula 1895) Castellani and Chalmers 1919 DSM 498 We strongly recommend to use only DSM 498 for propagation. |
Cultivation conditions: | Medium 1 , 37°C
Complete DSMZ Media List |
Phage propagation: | Supply, storage and propagation of phages Revitalisation of Vacuum-Dried Phages When stored at 4°C, the phage must be stabilized by addition of NaCl to a concentration of 1 M. Please use NZCYM medium for phage propagation (Medium 544). |
Delivery | |
Supplied as: | Delivery form | | Prices | | Vacuum Dried | | 160,- € | Liquid Suspension | | 160,- € | | Price Category for this culture: 6 |
Freight and handling charges will be added. See price list. |
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