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| ARCHAEA |  How to read the following data (Example) | |||||||||||||||||
| Name: | "Candidatus Nitrosotenuis aquarius" | |||||||||||||||||
| DSM No.: | 115850, Proposed type strain dsm-115850 dsm115850 dsm 115850 Candidatus Nitrosotenuis aquarius | |||||||||||||||||
| Strain designation: | AQ6F | |||||||||||||||||
| Isolated from: | Freshwater aquarium biofilter | |||||||||||||||||
| Country: | Canada Ontario, Waterloo, University of Waterloo | |||||||||||||||||
| Date of sampling: | 2021-12-01 | |||||||||||||||||
| Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. | |||||||||||||||||
| History: | <- J. Neufeld and A. Umbach; {2023} <- J. Neufeld and L. Sauder, Univ. Waterloo, Canada; AQ6F {2014} | |||||||||||||||||
| Genbank accession numbers: | complete genome: CP024808 16S rRNA gene: KX023321 BioProject: PRJNA406986 BioSample: SAMN07637255 amoA gene: KX034182 | |||||||||||||||||
| Cultivation conditions: | Medium 1845 , 28°C Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775). The delivery time is several months Complete DSMZ Media List | |||||||||||||||||
| Summary and additional information: | <- J. Neufeld and A. Umbach; {2023} <- J. Neufeld and L. Sauder, Univ. Waterloo, Canada; AQ6F {2014}. Published as "Candidatus Nitrosotenuis aquarius" Sauder et al. 2018. Proposed renaming to "Candidatus Nitrosotenuis aquariorum" Oren et al. 2020. Freshwater aquarium biofilter. Canada, Ontario, Waterloo, University of Waterloo. Taxonomy/description (37580, 40844). Sequence accession no. complete genome: CP024808, 16S rRNA gene: KX023321, BioProject: PRJNA406986, BioSample: SAMN07637255, amoA gene: KX034182. Chemolithoautotrophic. Oxidation of ammonia to nitrite. Risk Group 2: enrichment of Archaea with partly known bacteria (non-specific activity, German BioStoffV Section 2 point (8)), such as <i>Afipia</i> sp. (Risk Group 2, German TRBA 466). (Medium 1845, incubation time: more than 4 weeks, pH 8.4, 28°C, aerobic). Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775). The delivery time is several months | |||||||||||||||||
| Literature: | 37580, 40844, 41046 | |||||||||||||||||
| Risk group: | 2 (provisional classification by DSMZ) | |||||||||||||||||
| Restrictions: | Act dealing with the prevention & control of infectious diseases in man (Infektionsschutzgesetz), Category A1 | |||||||||||||||||
| Supplied as: |
Freight and handling charges will be added. See price list. Note: Freight charges for Risk Group 2 microorganisms will apply. | |||||||||||||||||
| Other cultures: | All DSMZ cultures of the species | |||||||||||||||||
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