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| ARCHAEA |  How to read the following data (Example) | |||||||||||||||||
| Name: | Nitrosopumilus adriaticus Bayer et al. 2019 | |||||||||||||||||
| DSM No.: | 106890, Type strain dsm-106890 dsm106890 dsm 106890 Nitrosopumilus adriaticus | |||||||||||||||||
| Strain designation: | NF5 | |||||||||||||||||
| Other collection no. or WDCM no.: | JCM 32270,NCIMB 15114 | |||||||||||||||||
| Isolated from: | coastal surface water | |||||||||||||||||
| Country: | Slovenia Northern Adriatic Sea | |||||||||||||||||
| Date of sampling: | 2011-11-23 | |||||||||||||||||
| Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. | |||||||||||||||||
| History: | <- C. Sedlazek and B. Bayer, University Vienna, Fac. of Life Scs, Vienna, Austria; NF5 {2018} <- B. Bayer; NF5 {2011} | |||||||||||||||||
| Genbank accession numbers: | complete genome: CP011070.1 | |||||||||||||||||
| Cultivation conditions: | Medium 1630a , 28°C Medium/media recipe(s) required for preparing 1630a: - Medium 141 - Medium 722 Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775). The delivery time is several months Incubation time: more than 14 days Complete DSMZ Media List | |||||||||||||||||
| Summary and additional information: | <- C. Sedlazek and B. Bayer, University Vienna, Fac. of Life Scs, Vienna, Austria; NF5 {2018} <- B. Bayer; NF5 {2011}. Coastal surface water. Slovenia, Northern Adriatic Sea. Type strain.. Taxonomy/description (32605). Sequence accession no. complete genome: CP011070.1. G+C content 33.4 mol%. Strain-specific literature (26775, 32586). (Medium 1630a, incubation time: more than 4 weeks, 28°C, aerobic). Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775). The delivery time is several months | |||||||||||||||||
| Literature: | 32605, 26775, 32586 | |||||||||||||||||
| Image: | ###IMAGE_TPL### Medium 1630a, 30°C, cell suspension concentrated by centrifugation | |||||||||||||||||
| Risk group: | 1 (classification according to German TRBA) | |||||||||||||||||
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| Other cultures: | All DSMZ cultures of the species | |||||||||||||||||
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