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Name: | Nitrosopumilus zosterae Nielsen et al. 1999 |
DSM No.: | 116344, Type strain dsm-116344 dsm116344 dsm 116344 Nitrosopumilus zosterae |
Strain designation: | NM25 |
Other collection no. or WDCM no.: | NBRC 111181,ATCC TSD-147 |
Isolated from: | sediment near eelgrass |
Country: | Japan Tanoura Bay, Shimoda, Shizuoka Prefecture |
Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. |
History: | <- NBRC; NBRC 111181 {2015} <- T. Nakagawa, Univ. Nihon; NM25 {2007} |
Genbank accession numbers: | 16S rRNA gene: LC571517 whole genome shotgun sequencing project: BGKI01000000 BioProject: PRJDB6801 BioSample: SAMD00113975 |
Cultivation conditions: | Medium 1630a , 28°C Medium/media recipe(s) required for preparing 1630a: - Medium 141 - Medium 722 or Medium 1630e add from sterile filtered stock solution 5 mL/L alpha-ketoglutamate (20 mM), 28°C
Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775)
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Summary and additional information: | <- NBRC; NBRC 111181 {2015} <- T. Nakagawa, Univ. Nihon; NM25 {2007}. Sediment near eelgrass. Japan, Tanoura Bay, Shimoda, Shizuoka Prefecture. Type strain.. Taxonomy/description (40347, 40841). Sequence accession no. 16S rRNA gene: LC571517, whole genome shotgun sequencing project: BGKI01000000, BioProject: PRJDB6801, BioSample: SAMD00113975. Oxidation of ammonia to nitrite. (Medium 1630a, incubation time: 7-14 days, 28°C, aerobic or Medium 1630e add from sterile filtered stock solution 5 mL/L alpha-ketoglutamate (20 mM), incubation time: 7-14 days, pH 7.5, 28°C, aerobic). Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775) |
Literature: | 40347, 40841, 40842, 40843 |
Image: | ###IMAGE_TPL### cells of DSM 116344 on medium 1630e at 28 °C Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH |
Risk group: | 1 (provisional classification by DSMZ) |
Supplied as: | Delivery form | | Prices | | actively growing culture | | 160,- € | | DNA | | 150,- € | | Price Category for this culture: 1 |
Freight and handling charges will be added. See price list. |
Other cultures: | All DSMZ cultures of the species |
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