| |
---|
| Detailed Price Information Link |
ARCHAEA | How to read the following data (Example) |
Name: | Nitrososphaera viennensis Stieglmeier et al. 2014 |
DSM No.: | 26422, Type strain dsm-26422 dsm26422 dsm 26422 Nitrososphaera viennensis |
Other collection no. or WDCM no.: | EN76,JCM 19564 |
Isolated from: | garden soil |
Country: | Austria Vienna, 9th, district |
Date of sampling: | 2008 |
Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. |
History: | <- M Melcher and C. Schleper, University Vienna, Fac. of Life Scs, Vienna, Austria; {2021} <- C. Schleper, University Vienna, Fac. of Life Scs, Vienna, Austria; EN76 {2016} <- M. Stieglmeier and C. Schleper; |
Genbank accession numbers: | 16S rRNA gene: FR773157 amoA gene: FR773159 amoB gene: FR773160 complete genome: CP007536 |
Cultivation conditions: | Medium 1630 , 40°C Medium/media recipe(s) required for preparing 1630: - Medium 141 - Medium 722 or Medium 1630c , (this medium is recommended for the routine cultivation of the strain), 40°C Medium/media recipe(s) required for preparing 1630c: - Medium 141 - Medium 320 - Medium 385
Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775)
Incubation time: more than 14 days
Complete DSMZ Media List |
Summary and additional information: | <- C. Schleper, Univ. Vienna, Fac. of Life Scs, Austria <- M. Stieglmeier and C. Schleper {2010}. Garden soil; Austria, Vienna, 9th, district (19786). Type strain. Taxonomy/description (19762). Sequence accession no. 16S rRNA gene: FR773157, amoA gene: FR773159, amoB gene: FR773160, complete genome: CP007536. Oxidizes ammonia to nitrite with oxygen as electron acceptor. Strain-specific literature (26775). (Medium 1554, 42°C). Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775). |
Literature: | 19762, 19786, 26775 |
Risk group: | 1 (classification according to German TRBA) |
Supplied as: | Delivery form | | Prices | | actively growing culture | | 240,- € | | Price Category for this culture: 3 |
Freight and handling charges will be added. See price list. |
Other cultures: | All DSMZ cultures of the species |
| Print data sheet |