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BACTERIA | How to read the following data (Example) |
Name: | Polynucleobacter wuianus Hahn et al. 2017 |
DSM No.: | 24008, Type strain dsm-24008 dsm24008 dsm 24008 Polynucleobacter wuianus |
Strain designation: | QLW-P1FAT50C-4, CIP 111100 |
Other collection no. or WDCM no.: | CIP 111100 |
Isolated from: | shallow acidic freshwater pond |
Country: | Austria Alps, Pond-1 (47° 44' 23.37'' N 13° 18' 6.03'' E) 1300 meters above sea level |
Date of sampling: | Oct. 2003 |
Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. |
History: | <- M. W. Hahn, Austrian Academy of Science, Inst. Limnology, Mondsee; QLW-P1FAT50C-4 <- Q. L. Wu, Austrian Academy of Sci., Inst. Limnology |
Genbank accession numbers: | complete genome: CP015922 16S rRNA gene plus ITS1: AJ879778 |
Cultivation conditions: | Medium 830 , 22°C or Medium 1167 , 22°C Medium/media recipe(s) required for preparing 1167: - Medium 14 - Medium 27
Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.
Complete DSMZ Media List |
Summary and additional information: | <- M. W. Hahn, Austrian Academy of Science, Inst. Limnology, Mondsee; QLW-P1FAT50C-4 <- Q. L. Wu, Austrian Academy of Sci., Inst. Limnology. Shallow acidic freshwater pond; Austria, Alps, Pond-1 (47° 44' 23.37'' N 13° 18' 6.03'' E) 1300 meters above sea level (10512, 26603). Type strain. Taxonomy/description (13477, 25987, 26603). Sequence accession no. complete genome: CP015922, 16S rRNA gene plus ITS1: AJ879778. (Medium 830, 22°C or Medium 1167, 22°C). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.. |
Literature: | 10512, 13477, 25987, 26603 |
Image: | ###IMAGE_TPL### © Leibniz-Institut DSMZ |
Risk group: | 1 (classification according to German TRBA) |
Supplied as: | Delivery form | | Prices | | actively growing culture | | 160,- € | | DNA | | 150,- € | | Price Category for this culture: 1 |
Freight and handling charges will be added. See price list. |
Other cultures: | All DSMZ cultures of the species |
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