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PHAGES | |
Species: | Escherichia phage phiX174 (Sinsheimervirus phiX174) |
Taxonomy: | Order: Petitvirales Family: Microviridae Subfamily: Bullavirinae Genus: Sinsheimervirus |
Isolate: | Escherichia phage phiX174 |
DSM No.: | 4497 dsm-4497 dsm4497 dsm 4497 Sinsheimervirus phiX174 |
History: | <- ATCC <- R.L. Sinsheimer |
Geographic origin: | country of origin unknown |
Date of sampling: | before 18.02.1993 |
Nagoya Protocol Restrictions: | There are NO known Nagoya protocol restrictions for this strain. |
Genomics: | ssDNA |
Morphology: | icosahedral capsid with 12 spikes, no tail, capsid size 25 nm |
Plaques description: | large plaques with a clear centre and turbid halo |
Image: | ###IMAGE_TPL### Single plaques on agar plate. Creator: B. Henze, Leibniz Institute DSMZ. © © Leibniz-Institut DSMZ |
Further information: | Prior to infection, the phage undergoes a conformational change and forms an eclipsed particle.
The phage does not produce high titers above 1E+8 pfu/mL. We recommend to perform lysis on plates, not in liquid culture |
Literature: | 6286, 15856, 23821 |
Risk group: | 1 |
Phage propagation | |
Host bacterium: | Escherichia coli (Migula 1895) Castellani and Chalmers 1919 DSM 13127 We strongly recommend to use only DSM 13127 for propagation. |
Cultivation conditions: | Medium 381 , 37°C
Complete DSMZ Media List |
Phage propagation: | Supply, storage and propagation of phages Revitalisation of Vacuum-Dried Phages |
Delivery | |
Supplied as: | Delivery form | | Prices | | Liquid Suspension | | 160,- € | | Price Category for this culture: 6 |
Freight and handling charges will be added. See price list. |
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