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PHAGES | |
Species: | Pseudomonas phage Psp4 |
Isolate: | Pseudomonas phage Psp4 |
DSM No.: | 9285 dsm-9285 dsm9285 dsm 9285 |
History: | <- J. Campbell-Tofte, Copenhagen |
Geographic origin: | country of origin unknown |
Date of sampling: | before 16.06.1994 |
Nagoya Protocol Restrictions: | There are NO known Nagoya protocol restrictions for this strain. |
Genomics: | 240 kb |
Morphology: | head diameter is 103 +/- 8 nm, extended tail length is 337 +/- 15 nm, appendages are tail fibres |
Life cycle: | virulent phage, lytic cycle |
Plaques description: | clear plaques are up to 1 mm in diameter |
Image: | ###IMAGE_TPL### Single plaques on agar plate. Creator: B. Henze, Leibniz Institute DSMZ. © © Leibniz-Institut DSMZ |
Further information: | For further general information see phage Psp1.
resistant to chloroform, < 2 minutes resistant to 60°C, the small burst size is 10 particles, the long latent period is 150 minutes. |
Literature: | 6305 |
Risk group: | 1 |
Phage propagation | |
Host bacterium: | Pseudomonas putida (Trevisan 1889) Migula 1895 DSM 9278 We strongly recommend to use only DSM 9278 for propagation. |
Cultivation conditions: | Medium 545 , 25°C
Complete DSMZ Media List |
Phage propagation: | Supply, storage and propagation of phages Revitalisation of Vacuum-Dried Phages Phage is not very productive, titer not higher than logE9 pfu/mL, plate propagation might be more efficient than liquid culture lysis. Optimal plate propagation at 25 °C for 2 days with 0.5% soft agar concentration. |
Delivery | |
Supplied as: | Delivery form | | Prices | | Liquid Suspension | | 160,- € | | Price Category for this culture: 6 |
Freight and handling charges will be added. See price list. |
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