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| DSM No.: | 113829 dsm-113829 dsm113829 dsm 113829 Acanthamoeba sp. |
| Strain designation: | Mono Lake KA, ML-KA, ML |
| Biotype: | T4 genotype |
| Isolated from: | Mono Lake (hypersaline) sediment |
| Country: | United States of America
Mono Lake, California |
| Date of sampling: | 2011-03-27 |
| Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. |
| History: | <- M. Horn, University of Vienna, Department of Microbiology and Ecosystem Science, Division of Microbial Ecology; Mono Lake KA {2011} <- K. Aistleitner, University of Vienna, Department of Microbiology and Ecosystem Science, Division of Microbial Ecology; |
| Genbank accession numbers: | partial 18S rRNA gene sequence: MH675534 |
| Cultivation conditions: | Medium 1779 , 20°C
- Maintenance conditions: Amoeba cultures can be maintained in 25 cm2 plastic tissue culture flasks equipped with filter screw caps at
20°C in an incubator (no special requirements concerning CO2 or light).
- Maintenance/Passage of amoeba cultures: Amoeba cultures can be maintained in the same 25 cm2 plastic tissue culture flasks for several weeks or months. However, if the cultures reach a high cell density (approximately each week), PYG medium should be exchanged. For that purpose amoebae are detached from the surface of the flask by knocking at the plastic tissue culture flasks (amoebae are only weakly adherent) and the medium (containing the majority of cells) is then completely removed. Fresh PYG medium (20 ml) is added and the few remaining attached amoebae will continue to grow.
Alternatively, cultures can also be passaged regularly (e.g. weekly) by transfer of a small aliquot of a densely grown culture (after cell detachment, as described above) to a fresh flask containing medium. The maximum final volume in 25 cm2 plastic tissue culture flasks is 20 ml.
If cultures contain few attached cells, but high amounts of cell debris, medium should be exchanged without prior cell detachment. Cultures usually recover fast.
- Storage: Cell cultures (supplemented with 10% DMSO) may be stored in liquid nitrogen.
Complete DSMZ Media List |
Summary and
additional information: | <- M. Horn, University of Vienna, Department of Microbiology and Ecosystem Science, Division of Microbial Ecology; Mono Lake KA {2011} <- K. Aistleitner, University of Vienna, Department of Microbiology and Ecosystem Science, Division of Microbial Ecology;. Mono Lake (hypersaline) sediment. United States of America, Mono Lake, California. Genotype T4. Strain-specific literature (41667). - Peptone-yeast-glucose medium (PYG): 20 g/l proteose peptone, 18 g/l glucose, 2 g/l yeast extract, 1 g/l sodium citrate dihydrate, 0.98 g/l MgSO4*7H2O, 0.355 g/l Na2HPO4*7H2O, 0.34 g/l KH2PO4, 0.02 g/l Fe(NH4)2(SO4)2*6H2O, pH 6.5, autoclave at 110°C
- Maintenance conditions: Amoeba cultures can be maintained in 25 cm2 plastic tissue culture flasks equipped with filter screw caps at
20°C in an incubator (no special requirements concerning CO2 or light).
- Maintenance/Passage of amoeba cultures: Amoeba cultures can be maintained in the same 25 cm2 plastic tissue culture flasks for several weeks or months. However, if the cultures reach a high cell density (approximately each week), PYG medium should be exchanged. For that purpose amoebae are detached from the surface of the flask by knocking at the plastic tissue culture flasks (amoebae are only weakly adherent) and the medium (containing the majority of cells) is then completely removed. Fresh PYG medium (20 ml) is added and the few remaining attached amoebae will continue to grow.
Alternatively, cultures can also be passaged regularly (e.g. weekly) by transfer of a small aliquot of a densely grown culture (after cell detachment, as described above) to a fresh flask containing medium. The maximum final volume in 25 cm2 plastic tissue culture flasks is 20 ml.
If cultures contain few attached cells, but high amounts of cell debris, medium should be exchanged without prior cell detachment. Cultures usually recover fast.
- Storage: Cell cultures (supplemented with 10% DMSO) may be stored in liquid nitrogen. |
| Literature: | 41667 |
| Risk group: | 2 (classification according to German TRBA) |
| Restrictions: | Act dealing with the prevention & control of infectious diseases in man (Infektionsschutzgesetz), Category A1
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| Supplied as: | | Delivery form | | Prices | | | actively growing culture | | 190,- € | | | Frozen | | 190,- € | | | DNA | | 180,- € | | | Price Category for this culture: 1 |
Freight and handling charges will be added. See price list.
Note: Freight charges for Risk Group 2 microorganisms will apply.
Note: Freight charges for frozen dispatch will apply. |
| Other cultures: | All DSMZ cultures of the species |
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