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CYANO | How to read the following data (Example) |
Name: | Coleofasciculus chthonoplastes (Thuret ex Gomont 1892) Siegesmund et al. 2008 |
DSM No.: | 104235 dsm-104235 dsm104235 dsm 104235 Coleofasciculus chthonoplastes |
Strain designation: | NCR |
Isolated from: | Intertidal habitat (marine) |
Country: | United States of America North Carolina, Rachel Carson Estuarine Reserve |
Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. |
History: | <- U. Karsten, Universität Rostock; NCR {2014} <- L. Prufert-Bebout and F. Garcia-Pichel; NCR {1993} |
Genbank accession numbers: | 16S + 23S partial sequence, intergenic spacer: EF654039.1 16S + 23S partial sequence, intergenic spacer: OR267348 16S + 23S partial sequence, intergenic spacer: OR267349 |
Cultivation conditions: | Medium 1831a , alternative medium: DSMZ Medium 1680, 23°C
At the DSMZ, the cyanobacterial cultures are usually cultivated without agitation in 25 cm2 plastic tissue culture flasks with filter screw caps. To subculture Coleofasciculus strains, scrape off the adhering cell material with a plastic Pasteur pipette and crush it by pipetting up and down several times. Alternatively, use a disposable pipette tip to dissect the cell material. Use 2-3 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask. Light cycle: 16 hours of illumination at 150-200 lux and 8 hours dark period. Transfer interval of 12 weeks.
Complete DSMZ Media List |
Summary and additional information: | <- U. Karsten, Universität Rostock; NCR {2014} <- L. Prufert-Bebout and F. Garcia-Pichel; NCR {1993}. Intertidal habitat (marine). United States of America, North Carolina, Rachel Carson Estuarine Reserve. Sequence accession no. 16S + 23S partial sequence, intergenic spacer: EF654039.1, 16S + 23S partial sequence, intergenic spacer: OR267348, 16S + 23S partial sequence, intergenic spacer: OR267349. Phototrophic. It cannot be excluded that the strain produces cyanobacterial toxins that can be dangerous to humans and pets. DSM 104235 is a non-axenic culture. Strain-specific literature (27474). (Medium 1831a, incubation time: more than 4 weeks, 23°C, alternative medium: DSMZ Medium 1680). At the DSMZ, the cyanobacterial cultures are usually cultivated without agitation in 25 cm2 plastic tissue culture flasks with filter screw caps. To subculture Coleofasciculus strains, scrape off the adhering cell material with a plastic Pasteur pipette and crush it by pipetting up and down several times. Alternatively, use a disposable pipette tip to dissect the cell material. Use 2-3 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask. Light cycle: 16 hours illumination at 150-200 lux and 8 hours dark period. Transfer interval of 12 weeks. |
Literature: | 27474 |
Risk group: | 1 (classification according to German TRBA) |
Supplied as: | Delivery form | | Prices | | actively growing culture | | 160,- € | | Price Category for this culture: 1 |
Freight and handling charges will be added. See price list. |
Other cultures: | All DSMZ cultures of the species |
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