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| CYANO |  How to read the following data (Example) | |||||||||||||
| Name: | "Coleofasciculus sp." | |||||||||||||
| DSM No.: | 104221 dsm-104221 dsm104221 dsm 104221 Coleofasciculus sp. | |||||||||||||
| Strain designation: | WW2 | |||||||||||||
| Isolated from: | Intertidal habitat (brackish, 10 PSU); wind flat | |||||||||||||
| Country: | Germany Baltic Sea, Zingst, Großer Werder | |||||||||||||
| Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. | |||||||||||||
| History: | <- U. Karsten, Universität Rostock, Germany; WW2 {2014} <- U. Karsten, Universität Rostock, Germany; {2002} | |||||||||||||
| Genbank accession numbers: | 16S ribosomal gene RNA, partial sequence: EF654051.1 16S + 23S partial sequence, intergenic spacer: OR339324 16S + 23S partial sequence, intergenic spacer: OR339325 | |||||||||||||
| Cultivation conditions: | Medium 1593 , alternative medium: DSMZ Medium 1680, 17°C At the DSMZ, the cyanobacterial cultures are usually cultivated without agitation in 25 cm2 plastic tissue culture flasks with filter screw caps. To subculture Coleofasciculus strains, scrape off the adhering cell material with a plastic Pasteur pipette and crush it by pipetting up and down several times. Alternatively, use a disposable pipette tip to dissect the cell material. Use 2-3 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask. Light cycle: 16 hours illumination at 300-350 lux and 8 hours dark period. Transfer interval of 12 weeks. Complete DSMZ Media List | |||||||||||||
| Summary and additional information: | <- U. Karsten, Universität Rostock, Germany; WW2 {2014} <- U. Karsten, Universität Rostock, Germany; {2002}. Intertidal habitat (marine). Germany, Baltic Sea, Zingst, Großer Werder. Taxonomy/description (27474). Sequence accession no. 16S + 23S partial sequence, intergenic spacer: EF654051.1, 16S + 23S partial sequence, intergenic spacer: OR339324, 16S + 23S partial sequence, intergenic spacer: OR339325. Phototrophic. It cannot be excluded that the strain produces cyanobacterial toxins that can be dangerous to humans and pets. DSM 104221 is a non-axenic culture. (Medium 1593, incubation time: more than 4 weeks, 17°C, alternative medium: DSMZ Medium 1680). At the DSMZ, the cyanobacterial cultures are usually cultivated without agitation in 25 cm2 plastic tissue culture flasks with filter screw caps. To subculture Coleofasciculus strains, scrape off the adhering cell material with a plastic Pasteur pipette and crush it by pipetting up and down several times. Alternatively, use a disposable pipette tip to dissect the cell material. Use 2-3 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask. Light cycle: 16 hours illumination at 300-350 lux and 8 hours dark period. Transfer interval of 12 weeks. | |||||||||||||
| Literature: | 27474 | |||||||||||||
| Risk group: | 1 (classification according to German TRBA) | |||||||||||||
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Freight and handling charges will be added. See price list. | |||||||||||||
| Other cultures: | All DSMZ cultures of the species | |||||||||||||
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