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| CYANO |  How to read the following data (Example) | |||||||||||||||||
| Name: | "Cyanocohniella crotaloides" | |||||||||||||||||
| DSM No.: | 109255, Type strain dsm-109255 dsm109255 dsm 109255 Cyanocohniella crotaloides | |||||||||||||||||
| Strain designation: | PJ S45 | |||||||||||||||||
| Other collection no. or WDCM no.: | SAG 2592 | |||||||||||||||||
| Isolated from: | beach mats formed of fine sand | |||||||||||||||||
| Country: | Netherlands North Sea barrier island Schiermonnikoog | |||||||||||||||||
| Date of sampling: | 2018-05-23 | |||||||||||||||||
| Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. | |||||||||||||||||
| History: | <- P. Jung, University of Applied Sciences Kaiserslautern, Campus Pirmasens; PJ S45 {2019} | |||||||||||||||||
| Genbank accession numbers: | 16S + 23S partial sequence, intergenic spacer: MN243143.1 | |||||||||||||||||
| Cultivation conditions: | Medium 1593 , 17°C At the DSMZ, cyanobacterial cultures are usually cultivated without agitation in 25 cm² plastic tissue culture flasks with filter screw caps. To subculture DSM 109255, scrape off the adhering cell material with a plastic Pasteur pipette, then crush it by pipetting up and down several times. Use 1–2 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask. The light cycle is 16 hours of illumination at 800 lux and an 8-hour dark period. The transfer interval is six weeks. Complete DSMZ Media List | |||||||||||||||||
| Summary and additional information: | <- P. Jung, University of Applied Sciences Kaiserslautern, Campus Pirmasens; PJ S45 {2018}. Beach mats formed of fine sand. Netherlands, North Sea barrier island Schiermonnikoog. Type strain.. Taxonomy/description (40106, 40107). Sequence accession no. 16S + 23S partial sequence, intergenic spacer: MN243143.1. Phototrophic. It cannot be excluded that the strain produces cyanobacterial toxins that can be dangerous to humans and pets. DSM 109255 is a non-axenic culture. (Medium 1593, incubation time: more than 14 days, pH 7, 17°C). At the DSMZ, the cyanobacterial cultures are usually cultivated without agitation in 25 cm2 plastic tissue culture flasks with filter screw caps. To subculture DSM 109255 scrape off the adhering cell material with a plastic Pasteur pipette and crush it by pipetting up and down several times. Use 1-2 ml of the shredded biomass to inoculate 15 ml of fresh medium in a new tissue culture flask. Light cycle: 16 hours of illumination at 800 lux and 8 hours dark period. Transfer interval of 6 weeks. | |||||||||||||||||
| Literature: | 40106, 40107 | |||||||||||||||||
| Risk group: | 1 (classification according to German TRBA) | |||||||||||||||||
| Supplied as: |
Freight and handling charges will be added. See price list. | |||||||||||||||||
| Other cultures: | All DSMZ cultures of the species | |||||||||||||||||
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