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DSM No.: | 114853 dsm-114853 dsm114853 dsm 114853 Ministeria vibrans |
Strain designation: | L27 |
Isolated from: | oxygen-depleted water at station Landsort Deep (deepest point of the Baltic sea also known as Landsortsdjupet), depth 105 m, salinity 10 |
Country: | Sweden Baltic Sea |
Date of sampling: | 15.06.2012 |
Nagoya Protocol Restrictions: | There are NO known Nagoya Protocol restrictions for this strain. |
History: | <- C. Wylezich, Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, Greifswald, Insel Riems; L27 {2012} |
Genbank accession numbers: | 18S rRNA gene: KX420739 |
Cultivation conditions: | Medium 1723a , 13°C
DSM 114853 is cultivated in 25 cm2 plastic tissue culture flasks with filter screw caps, routinely containing 20 ml of culture each. In order to establish a fresh culture of DSM 114853, fill a 25 cm2 plastic tissue culture flasks with 10 ml of DSM 1723a medium and inoculate with 10 ml of a grown DSM 114853 culture. Each T25 culture is aseptically supplemented with a sterilized wheat grain (autoclaved at 121 °C for 20 min) as carbon source for ambient bacteria from the sampling site. Transfer interval of 12 weeks.
Complete DSMZ Media List |
Summary and additional information: | <- C. Wylezich, Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, Greifswald, Insel Riems; L27 {2012}. Oxygen-depleted water at station Landsort Deep (deepest point of the Baltic sea also known as Landsortsdjupet), depth 105 m, salinity 10. Sweden, Baltic Sea. Sequence accession no. 18S rRNA gene: KX420739. Heterotrophic. Non-axenic. Strain-specific literature (40421, 40420). (Medium 1723a, incubation time: more than 14 days, 13°C, aerobic). DSM 114853 is cultivated in 25 cm2 plastic tissue culture flasks with filter screw caps, routinely containing 20 ml of culture each. In order to establish a fresh culture of DSM 114853, fill a 25 cm2 plastic tissue culture flasks with 10 ml of DSM 1723a medium and inoculate with 10 ml of a grown DSM 114853 culture. Each T25 culture is aseptically supplemented with a sterilized wheat grain (autoclaved at 121 °C for 20 min) as carbon source for ambient bacteria from the sampling site. Transfer interval of 12 weeks.
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Literature: | 40421, 40420 |
Risk group: | 1 (provisional classification by DSMZ) |
Supplied as: | Delivery form | | Prices | | actively growing culture | | 160,- € | | DNA | | 150,- € | | Price Category for this culture: 1 |
Freight and handling charges will be added. See price list. |
Other cultures: | All DSMZ cultures of the species |
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