| | |
|---|
| Cell line: | 17.94 |
| DSMZ no.: | ACC 741 |
| Species: | human (Homo sapiens) |
| Cell type: | Wilms´ tumor |
| Origin: | established from the nephrectomy specimen of a large lobulated Wilms´ tumor of a 4-year-old girl; cells were described in the literature to express NCAM, SALL1, and CITED1, and to contain a TP53 mutation |
| Reference(s): | 18006 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | pleomorphic epithelioid cells growing adherently in monolayers; the cytoplasm of the cells exhibits numerous granules; image; image; image; image; image |
| Medium: | 80% DMEM (with 4.5 g/l glucose) + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1 x 106 cells/25 cm2 flask; cells grow slowly and may need 1-2 days for adherence to the plastic surface;
split confluent culture 1:2 once a week using trypsin/EDTA (wash cells twice with PBS before adding trypsin/EDTA) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 1 week |
| Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage: | frozen in serum-free freezing medium (BambankerTM) |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
