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| Cell line: | 1G1 |
| DSMZ no.: | ACC 424 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-chicken dorsal retina cytoplasmic membranes, E12) |
| Origin: | cell line was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse immunized with enriched retinal cell membranes; described as secreting a mouse monoclonal antibody (IgM) reacting with the chicken dorsal retina cytoplasmic membranes (E12) and binding outer photoreceptor elements of adult vertebrate retina (inclusive chick, mouse, rat) |
| Reference(s): | 14941 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | spheroidal cells growing singly in suspension, or occasionally loosely adherent; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture: | maintain at ca. 0.1-0.3 x 106 cells/ml; split saturated culture 1:3 to 1:10 every 2-5 days (some cells attach to the surface of the culture flask, but can be detached by tapping the flask); seed out at ca. 0.3 x 106 cells/ml; a maximal density of 1.0 x 106 cells/ml should not be exceeded |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | secretion of a IgM monoclonal antibody |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine flat-moded hypertetraploid karyotype - 94-102<4n> - no centric fusion markers present |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
