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Cell line: | 23132/87 |
DSMZ no.: | ACC 201 |
Species: | human (Homo sapiens) |
Cell type: | gastric adenocarcinoma |
Origin: | established from the primary tumor of a 72-year-old man with gastric adenocarcinoma in 1987 |
Reference(s): | 15047 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like cells growing adherently as monolayer; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out with 2-3 x 106/80 cm2 flask; split confluent culture up to 1:8 every 5-6 days using trypsin/EDTA; initially after thawing, the viability might decrease down to ca. 20-50% during the first week and cells may grow slowly and loosely adherent, but cells recover subsequently over the next week and viability will raise over the next few passages |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 50 hours |
Harvest: | cell harvest of 10-12 x 106 cells/80cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertetraploid karyotype with 12% polyploidy - 47(45-52)<2n>XY, +20, t(1;15)(p11;p11), t(6;12) (p21;q21), i(13q), t(13;14)(p10;q10) - closely resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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