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Cell line: | 293 |
DSMZ no.: | ACC 305 |
Species: | human (Homo sapiens) |
Cell type: | embryonal kidney |
Origin: | established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) but not as GMO; cell line also known as HEK-293 (human embryonic kidney-293) |
Reference(s): | 14573 |
Biosafety level: | 1 |
Risk assessment: | The cell line was established by transfection of the cells with sheared DNA of adenovirus type 5. The cells contain 4 to 5 copies of the left end of the virus (12% of the viral genome) including E1a and E1b genes and one copy of the right end of the virus (10% of the genome) including the E4 gene. No active viruses are produced. No GMO. |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent fibroblastoid cells growing as monolayer; image; image; image; image |
Medium: | 90-95% DMEM (with 25mM HEPES) + 5-10% h.i. FBS |
Subculture: | seed out at about 2-3 x 106 cells/25 cm2; split confluent culture 1:5 to 1:6 every 2-3 days (with using trypsin/EDTA, cells can be detached by tapping the flask) |
Incubation: | at 37 °C with 5-10% CO2 |
Doubling time: | about 24-30 hours |
Harvest: | cell harvest of about 20 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-triploid karyotype with 6% polyploidy - 69(64-69)XX?X, +1, +6, -10, -13, -16, -18, -20, +21, +22, +mar, add(?X)(p22), inv(1)(p35q43), del(1)(p35), i(5p), del(6)(q11), add(8)(q24), add(13)(p13), add(17)(qter), der(?)t(5;?)(q13;?) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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