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Cell line: | 293T |
DSMZ no.: | ACC 635 |
Species: | human (Homo sapiens) |
Cell type: | embryonal kidney |
Origin: | highly transfectable derivative of the human primary embryonal kidney cell line 293 (ACC 305) carrying a plasmid containing the temperature sensitive mutant of SV-40 large T-antigen (tsA1609); cells originally referred to as 293tsA1609neo; cells were described in the literature to be Geniticin (Neomycin, G418) resistant and to express SV-40 large T at 33°C, but not at 40°C; classified as risk group 1 according to the German Central Commission for Biological Safety (ZKBS) |
Reference(s): | 14893, 14495, 14839 |
Biosafety level: | 1, GMO-S1 |
Risk assessment: | The cell line was established by transfection of the cells with sheared DNA of adenovirus type 5. The cells contain 4 to 5 copies of the left end of the virus (12% of the viral genome) including E1a and E1b genes and one copy of the right end of the virus (10% of the genome) including the E4 gene. No active viruses are produced. |
Permissions and restrictions: | A, D |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | oval to fibroblastoid cells growing adherently as monolayer; image; image; image; image |
Medium: | 90% DMEM + 10% h.i. FBS |
Subculture: | seed out at ca. 1-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days (without using trypsin/EDTA, cells can be detached by tapping the flask) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 24-30 hours |
Harvest: | cell harvest of ca. 40 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded near-triploid karyotype; 61-73<3n>XXX, add(1)(p32), add(1)(q44)x1-2, der(4;10)(q10;q10), del(8)(p12), t(9;9)(p24;q21), der(13)t(6;13)(q21;q32), der(14)t(13;14)(q32;q32), del(17)(p11), der(22)t(17;22)(q21;q22) |
Molec. Genetics: | presence of large T-antigene (SV40) was confirmed by genomic PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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