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| Cell line: | 2A1 |
| DSMZ no.: | ACC 281 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-chicken retina) |
| Origin: | established by fusion of mouse NS-1 cells with spleen from a BALB/c ByI mouse immunized with White Leghorn chicken embryonic retinal and tectal membranes; secretes 2A1 monoclonal antibody which binds epitopes involved in chicken retinal morphogenesis |
| Reference(s): | 14938 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly in suspension or loosely adherent; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1-2 weeks post-thawing |
| Subculture: | cells are difficult to culture! maintain at ca. 0.05-0.5 x 106 cells/ml; split saturated culture 1:4 to 1:8 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 25 hours |
| Harvest: | maximal density at ca. 0.7 x 106 cells/ml (which should not be exceeded) |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | secretion of a IgM monoclonal antibody |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypertetraploid karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
