| |
---|
Cell line: | 32D |
DSMZ no.: | ACC 411 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | bone marrow |
Origin: | established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent |
Reference(s): | 14575, 14763, 15034 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent; image1; image2 |
Medium: | 90% RPMI 1640 + 10% h.i. FBS + 10-20% vol conditioned medium of cell line WEHI-3B (DSM ACC 26) |
Subculture: | maintain at 0.02-1.5 x 106 cells/ml; split 1:10 to 1:50 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 15 hours |
Harvest: | maximum density at ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD11b +, F4/80 (+); image |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine hypodiploid karyotype - 35(34-35)<2n> - carries 5 centric fusion markers |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |