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| Cell line: | 8505C |
| DSMZ no.: | ACC 219 |
| Species: | human (Homo sapiens) |
| Cell type: | thyroid carcinoma |
| Origin: | established from the primary tumor of a 78-year-old woman with thyroid carcinoma (undifferentiated carcinoma, histologically a largely papillary adenocarcinoma with some spindle, polygonal and giant cells) |
| Reference(s): | 14631 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like adherent cells growing as monolayer; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:5 every 2-3 days or 1:10 every 4-5 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 30-40 hours |
| Harvest: | cell harvest of about 20 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 16% polyploidy - 65(57-68)<3n>XX, -X, -2, +3, -7, -10, +11, -13, -16, -18, -19, +2mar, del(1)(q13), del(3)(q22), ?del(4)(q21), i(8q) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
