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Cell line: | 8709 |
DSMZ no.: | ACC 588 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | acute myeloid leukemia |
Origin: | established from the splenocytes of a radiation-induced acute myeloid leukemia (AML) of the C3H inbred mouse strain in 1997 |
Reference(s): | 14534, 15205 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | cells growing singly or in small clusters in suspension, partly slightly adherent |
Medium: | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine + 10 ng/ml muIL-3 or 10-20% WEHI-3B supernatant |
Subculture: | split culture 1:2 twice a week; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-1.0 x 106 cells/ml; after thawing it is advisable to culture the cells first in 24-well plates and to add 10 ng/ml mIL-3 or 10-20% WEHI-3B supernatant daily; viability may be low after thawing |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 60-80 hours |
Harvest: | maximum density at about 1.2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Viruses: | PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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