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| Cell line: | A-2 |
| DSMZ no.: | ACC 276 |
| Species: | fish - Poeciliid (Xiphophorus xiphidium) |
| Cell type: | non-tumorous embryonal cells |
| Origin: | derived from non-tumorous epithelial-like embryonal cells of the sweetwater poeciliid fish (platyfish) Xiphophorus xiphidium; origin: Rio Purificacion; strain: YF1 |
| Reference(s): | 14695, 14750 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | grows as single cells or in clumps in suspension; loosely adherent at times; image; image; image |
| Medium: | 80-85% mixture of Ham's F12 + DMEM (at 1:1) + 15-20% h.i. FBS
or: 80-90% Medium 199 + 10-20% h.i. FBS |
| Subculture: | split culture 1:3 about 2-3 times per week before cells reach confluency; cells grow loosely adherent and can be detached from the plastic surface by shaking or pipetting; old cultures tend to form foci and lose adherence, but the cells remain attached to each other and are difficult to disperse |
| Incubation: | with Ham's F12 + DMEM: at 26-28 °C with 10% CO2;
with Medium 199: at 26-28 °C without CO2 |
| Doubling time: | about 2-3 days |
| Harvest: | maximal density of 6-10 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Xiphophorus xiphidium species. |
| Viruses: | ELISA: reverse transcriptase negative |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
