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| Cell line: | A-549 |
| DSMZ no.: | ACC 107 |
| Species: | human (Homo sapiens) |
| Cell type: | lung carcinoma |
| Origin: | established from an explanted lung tumor which was removed from a 58-year-old man in 1972; model for alveolar type II pulmonary epithelium; cells were originally described in the literature to induce tumors in athymic mice; later described as squamous-type carcinoma; widely used and extensively characterized cell line (see ref 40398 and Cellosaurus:Expasy); listed in the NCI cancer cell line panel and in the CCLE (ref 18232) |
| Reference(s): | 14556, 14717, 40398, 18232, 40404 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial cells, growing adherently as monolayer; image; image; image; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split confluent cultures 1:5 to 1:10 every 4-7 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-30 hours |
| Harvest: | about 20 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 8% polyploidy - 65(59-66)<3n>XXY, -1, -3, -6, +12, -13, -15, -18, -19, -21, -22, +4mar, der(6)t(1;6)(q11;q27), del(11)(q22-23) - presence of large distinctive der(6) marker confirms identity of this cell line |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
