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| Cell line: | A4-1025 |
| DSMZ no.: | ACC 296 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-fiber/myosin) |
| Origin: | described as secreting a mouse monoclonal antibody (IgG2a) detecting all fibers in skeletal muscle and myosin heavy chains on Western blots and detecting mammalian, chicken, zebrafish, axolotl, xenopus skeletal muscle myosin heavy chains; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin |
| Reference(s): | 14444 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round single cells in suspension, partly in clusters, lightly adherent; image; image; image |
| Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids |
| Subculture: | split saturated culture 1:5 to 1:10 every 2-3 days; seed out at about 1.0-1.5 x 105 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 40-50 hours |
| Harvest: | cell harvest of about 0.5-1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | secretion of a IgG2a monoclonal antibody |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypertetraploid karyotype with 5% polyploidy - 84(79-84) - one large submetacentric and up to 5 centric fusion markers present |
| Viruses: | ELISA: reverse transcriptase positive |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
