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Cell line: | AC-1M81 |
DSMZ no.: | ACC 456 |
Species: | human (Homo sapiens) |
Cell type: | choriocarcinoma-trophoblast hybrid |
Origin: | one of five phenotypically and cytogenetically distinct subclones of a hybrid (ACH1P, DSMZ ACC 481) formed by fusion of the JEG-3-derived HGPRT-defective mutant cell line AC1-1 with primary male trophoblast cells isolated from term human chorion laeve; described as possessing both, a distinct karyotype and a distinct expression pattern of tumor-related genes |
Reference(s): | 14543 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like adherent cells growing in monolayers |
Medium: | 90% Ham's F12 + 10% h.i. FBS |
Subculture: | split confluent culture 1:3 to 1:5 twice a week using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2 |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 24 hours |
Harvest: | cell harvest of ca. 0.4 x 106 cells/cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with sparfloxacin, then negative in microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | near identical DNA profiles shared by the AC-sister cell lines, partly identical with JEG-3 and BEWO |
Cytogenetics: | human flat-moded hypertetraploid karyotype with 4% polyploidy - 80-106<4n>XX, -Y, -3, -4, +7, +9, -13, -18, +22, +4-6mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32)x2, i(13q), add(15)(p13), add(19)(p13), der(19)t(11;19)(q11;q13) - resembles published karyotype and those of sisters with minor, though consistent, differences (mirroring DNA profiles) |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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