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| Cell line: | AM-HLH |
| DSMZ no.: | ACC 908 |
| Species: | human (Homo sapiens) |
| Cell type: | Hodgkin lymphoma |
| Origin: | established in 2013 from the pleural fluid of a 65-year old male with EBV+ nodular sclerosis Hodgkin lymphoma; cells are described to secrete IL-10 and carry rearranged immunoglobuline genes; karyotype analysis including FISH are provided in the original paper |
| Reference(s): | 40210, 40211, 40388 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2. |
| Permissions and restrictions: | A, B |
| | DSMZ Cell Culture Data: |
| Morphology: | small, round cells growing singly in suspension; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out and maintain at 0.5-1 x 106 cells/ml; split 1:2 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 48 hours |
| Harvest: | maximal density of ca. 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD2 +, CD3 -, CD4 -, CD5 -, CD8 -, CD10 -, CD13 -, CD14 -, CD15 -, CD19 -, CD30 +, CD33 -, CD34 -, CD38 +, CD71 +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | IL-10 expression was verified by RT-PCR |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
