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| Cell line: | AMO-1 |
| DSMZ no.: | ACC 538 |
| Species: | human (Homo sapiens) |
| Cell type: | plasmacytoma |
| Origin: | established from the ascitic fluid of a 64-year-old woman with plasmacytoma (IgAkappa) of the duodenum two months following resection of the tumor mass (additional therapy not specified) in 1984; plasmacytoma is related to multiple myeloma; described to express CD4 |
| Reference(s): | 14956, 14486 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly in suspension; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.8 x 106 cells/ml; maintain at ca. 0.5-1.2 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days; minimum density at ca. 0.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 70-90 hours |
| Harvest: | maximum cell density of ca. 1.8 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, cy IgG -, cy IgM -, cy kappa +, cy lambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
