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| Cell line: | B-16V |
| DSMZ no.: | ACC 370 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | melanoma |
| Origin: | established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice |
| Reference(s): | 14996 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epithelial cells growing in monolayers; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1.5-2.0 x 106 cells/80 cm 2; split semi-confluent culture up to 1:10 about every 3 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20 hours |
| Harvest: | cell harvest of ca. 35 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine karyotype |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
